|
|
|
Molecular Biology
|
Molecular Biology & Genetic engineering Molecular Biology & Genetic engineering Department started its scientific activity by establishment of Stem Cell Technology Company in 2005.The main aims of our department are molecular biology studying and gene manipulation of stem cells along with providing molecular techniques required for other departments of the company. For these purposes, this department not only has extensively made use of young genius researchers but also has benefited from invaluable consultations with experts in stem cell biology.
Moreover, there has been creative collaboration between our center and national and International centers. These collaborations have brought brilliant results for not only our company but also for scientific community.
Currently, the Molecular biology and genetic engineering department has focused on particular research subjects that some of them have been described in detail as follows:
1. microRNA detection and quantification:
MicroRNAs regulate expression of their target mRNA through translation repression. Because of their short length, MicroRNAs are difficult to detect. We aimed to develop a method that could profile the expression of several MicroRNAs simultaneously by combining ligation of sized-coded probes and PCR amplification. The new method is able to distinguish between MicroRNAs that differ by only one nucleotide and also detects only mature MicroRNAs. Using this method, we generated unique MicroRNAs signatures for various cell and tissue samples.
2. Design and construction of tissue specific vector
Expression vectors have a wide range of application in biomedical researches. In filed of stem cell biology, vectors that express reporter genes like green florescent protein or GFP are powerful tools for monitoring the process of differentiation. Among our main objective in this department is design and construction of reporter vector in order to be used in our basic research. Currently, we are running a project in which we want to construct a neuron specific vector in order to monitor the differentiation of adult or embryonic stem cell to neurons.
3. MicroRNA profiling of mouse tissues and stem cells during aging
By Using a novel, simple and effective method which has been devised and optimized by our reserach team, we have been able to determine the expression profile of several miRNAs in tissue like bone marrow, brain and live of mouse. we are studying the diffrences in miRNA profile of two groups of young and old animals so that we could find those molecules which play crucial role in longevity and aging.
In this study our main aim is to find that microRNA which might be down-regulated or up-regulated during aging. Afterwards, we are going to manipulate microRNA involved in aging process in order to find key molecules which are responsible for aging.
4. Differentiation of stem cells through genetic manipulation
Alternative methods for differentiation of stem cells are a necessity in biology of these versatile cells. Differentiation factors induce cells to differentiate into a particular cell type by externally activating signaling pathways of cells. In an attempt to develop new method for differentiation of cells, we are trying to genetically manipulate cells and interfere with signaling pathways of cells. Therefore, we could determine the fate of cells by interfering with pathways which play important role in cell differentiation. |
| |
|
|
Email
To check emails , please click on the bellow icon 
|
